Dual oxidative stress and fatty acid profile impacts in Paracentrotus lividus exposed to lambda-cyhalothrin: biochemical and histopathological responses.

Lambda-cyhalothrin (λ-cyh) is a potential pyrethroid insecticide widely used in pest control. The presence of pyrethroids in the aquatic ecosystem may induce adverse effects on non-target organisms such as the sea urchin. This study was conducted to assess the toxic effects of λ-cyh on the fatty acid profiles, redox status, and histopathological aspects of Paracentrotus lividus gonads following exposure to three concentrations of λ-cyh (100, 250 and 500 µg/L) for 72 h. The results showed a significant decrease in saturated fatty acid (SFAs) with an increase in monounsaturated fatty acid (MUFAs) and polyunsaturated fatty acid (PUFAs) levels in λ-cyh treated sea urchins. The highest levels in PUFAs were recorded in the eicosapentaenoic acids (C20:5n-3), docosahexaenoic acids (C22:6n-3) and arachidonic acids (C20:4n-6) levels. The λ-cyh intoxication promoted oxidative stress with an increase in hydrogen peroxide (H2O2), malondialdehyde (MDA) and advanced oxidation protein products (AOPP) levels. Furthermore, the enzymatic activities and non-enzymatic antioxidants levels were enhanced in all exposed sea urchins, while the vitamin C levels were decreased in 100 and 500 µg/L treated groups. Our biochemical results have been confirmed by the histopathological observations. Collectively, our findings offered valuable insights into the importance of assessing fatty acids' profiles as a relevant tool in aquatic ecotoxicological studies.

Variations in nutritional quality and fatty acids composition of sardine (Sardina pilchardus) during canning process in grape seed and olive oils.

Fish canning industries generally use different oils to ensure the juicing stage of canned sardines. In this context, we tested the use of grape seed oil (GSO) which could provide several health benefits to consumers. This study compared its effects on the quality of canned sardine to that of olive oil (OO). Total polyphenols, flavonoids and non flavonoids of the tested GSO were significantly higher than those of the OO. Also, The GSO was rich in polyunsaturated fatty acid (PUFA), namely linoleic acid (65.36% of total fatty acids). The use of GSO in the sardine sardines canning process increased significantly fat, protein and ash contents after 90 days of conservation. The fatty acid profile was dominated by PUFA for all the tested samples. Docosahexaenoic acid was the most abundant unsaturated fatty acid, followed by linoleic acid in GSO samples (20.86 ± 0.06% compared to 1.46 ± 0.05% in fresh sardine) and oleic acid in OO samples. Both atherogenic and thrombogenic indices decreased after the canning process in OO and GSO to less than 1. Thus GSO seems to improve the lipid nutritional quality in fresh sardine. In addition, the values for thiobarbituric acid and Total volatile base nitrogen did not exceed critical limits.

Optimization of fishing for stock enhancement of Rutilis rutilus and Scardinius erythropththalmus in forage fish-deficient Tunisian reservoirs.

Twenty specially designed fishing nets were used to catch broodstock of the forage fish roach and rudd (Rutilus rutilus and Scardinius erythrophthalmus). The monofilament gill nets were of different mesh sizes (18, 22, 24, and 26 mm) but of identical dimensions (4 × 50 m). The fish, caught in Sidi Salem reservoir in 2014, amounted to a total of 8901 roach and rudd caught at 41 different times; 3335 broodstock forage fish were transferred to 11 forage fish-deficient Tunisian reservoirs. The best yields (0.464 fish/m2) were obtained with fishing gear fitted with 18-mm mesh size; however, fish mortality with this net was very high, exceeding 83.36%. The greatest efficiency was obtained using nets of 22- and 24-mm mesh with a number of fish per unit effort (NPUE) of 0.181 and 0.173 fish/m2, and a mortality of 54.34 and 53.85%, respectively. Gill nets of 26-mm mesh size were inappropriate (lowest yield 0.129 fish/m2). Length of rudd and roach at first maturity and Gonadosomatic index (GSI) were measured, indicating that all transferred fish were mature. Transfer techniques were improved by holding the captured fish for 5 days before release into the host reservoir.

Characterization of fish assemblages and population structure of freshwater fish in two Tunisian reservoirs: implications for fishery management.

To monitor and assess the state of Tunisian freshwater fisheries, two surveys were undertaken at Ghezala and Lahjar reservoirs. Samples were taken in April and May 2013, a period when the fish catchability is high. The selected reservoirs have different surface areas and bathymetries. Using multi-mesh gill nets (EN 14575 amended) designed for sampling fish in lakes, standard fishing methods were applied to estimate species composition, abundance, biomass, and size distribution. Four species were caught in the two reservoirs: barbel, mullet, pike-perch, and roach. Fish abundance showed significant change according to sampling sites, depth strata, and the different mesh sizes used. From the reservoir to the tributary, it was concluded that fish biomass distribution was governed by depth and was most abundant in the upper water layers. Species size distribution differed significantly between the two reservoirs, exceeding the length at first maturity. Species composition and abundance were greater in Lahjar reservoir than in Ghezala. Both reservoirs require support actions to improve fish productivity.